Paper of the month

A cell cycle-dependent CRISPR-Cas9 activation system based on an anti-CRISPR protein shows improved genome editing accuracy

Highly Parallel Profiling of Cas9 Variant Specificity

A mouse model of SARS-CoV-2 infection and pathogenesis

A Genome-Editing Nanomachine Constructed with a Clustered Regularly Interspaced Short Palindromic Repeats System and Activated by Near-Infrared Illumination CRISPR/Cas9 is a novel genome editing tool, that consists of…

Effective CRISPR interference of an endogenous gene via a single transgene in mice

Expanding the editable genome and CRISPR–Cas9 versatility using DNA cutting-free gene targeting based on in trans paired nicking Xiaoyu Chen, Francesca Tasca, Qian Wang, Jin Liu, Josephine M. Janssen, Marcella D.…

Cancer therapy with a CRISPR-assisted telomerase-activating gene expression system Wei Dai, Xinhui Xu, Danyang Wang, Jian Wu, Jinke Wang

Improved Cas9 activity by specific modifications of the tracrRNA Tristan Scott, Ryan Urak, Citradewi Soemardy & Kevin V. Morris

Search-and-replace genome editing without double-strand breaks or donor DNA Andrew V. Anzalone, Peyton B. Randolph, Jessie R. Davis, Alexander A. Sousa, Luke W. Koblan, Jonathan M. Levy, Peter J. Chen, Christopher…

Search-and-replace genome editing without double-strand breaks or donor DNA Anzalone AV1,2,3, Randolph PB1,2,3, Davis JR1,2,3, Sousa AA1,2,3, Koblan LW1,2,3, Levy JM1,2,3, Chen PJ1,2,3, Wilson C1,2,3, Newby GA1,2,3,…

Super-Mendelian inheritance mediated by CRISPR-Cas9 in the female mouse germline Hannah A. Grunwald1,5, Valentino M. Gantz1,5, Gunnar Poplawski2,4,5, Xiang-Ru S. Xu1, Ethan Bier1,3 and Kimberly L. Cooper1,3*

Improving CRISPR-Cas9 Genome Editing Efficiency by Fusion with Chromatin-Modulating Peptides

Improved CRISPR-Cas12a-assisted one-pot DNA editing method enables seamless DNA editing

Development of hRad51-Cas9 nickase fusions that mediate HDR without double-stranded breaks.

In utero gene editing for monogenic lung disease

Long-term evaluation of AAV-CRISPR genome editing for Duchenne muscular dystrophy

Engineering of CRISPR-Cas12b for human genome editing

Predictable and precise template-free CRISPR editing of pathogenic variants

Covalent linkage of the DNA repair template to the CRISPRCas9 nuclease enhances homology-directed repair

CRISPR-SKIP: Programmable gene splicing with single base editors

Repair of double-strand breaks induced by CRISPR-Cas9 leads to large deletions and complex rearrangements.

CRISPR-Cas9 genome editing induces a p53-mediated damage response

T315I mutation of BCR-ABL1 into human Philadelphia chromosome-positive leukemia cell lines by homologous recombination using the CRISPR/Cas9 system.

Partial DNA-guided Cas9 enables genome editing with reduced off-target activity

Tild-CRISPR Allows for Efficient and Precise Gene Knockin in Mouse and Human Cells.

Evolved Cas9 variants with broad PAM compatibility and high DNA specificity